Journal: PLoS ONE

Article Title: The Differential Interaction of Brucella and Ochrobactrum with Innate Immunity Reveals Traits Related to the Evolution of Stealthy Pathogens

doi: 10.1371/journal.pone.0005893

Figure Lengend Snippet: (A) Neutrophils were depleted from twenty mice by injection of the anti-neutrophil RB6 antibody and ten additional mice were instead injected with PBS alone. One group of ten mice anti-neutrophil treated and the group of ten mice injected with PBS alone were then infected intraperitoneally with 10 9 CFU/mouse of O. anthropi . The last group of ten mice treated with antibody anti-neutrophil was inoculated with PBS alone. The lethality of the bacteria was recorded at the indicated times. (B) O. anthropi was mixed with purified rat neutrophils at a ratio of 5±4 bacteria/cell. Control bacteria were not incubated with neutrophils. At the indicated times the viable CFU were determined and the percentage of bacterial replication was calculated. In “B” Values of p<0.01 (*) and p<0.001 (**) are indicated.

Article Snippet: After rinsing, only hMD-2 without LPS was detected by incubation with 0.1 µg/ml of mouse monoclonal anti-hMD-2 (clone 9B4 e-Bioscience San Diego, CA., U.S.A.) in PBS buffer at 37°C for 1 h, followed by incubation with 0.1 µg/ml peroxidase-conjugated goat anti-mouse IgG (Santa Cruz, CA., U.S.A.), also in PBS buffer at 37°C for 1 h. After plate washing, ABTS (Sigma) was added, the reaction was stopped with 1% SDS after 15 min, and the absorbance at 420 nm measured using a Mithras LB940 apparatus. (Berthold Technologies).

Techniques: Injection, Infection, Purification, Incubation